| Abstract |
For the purpose of investigating the possibility of hyperthermic therapy
against choriocarcinoma, an in vitro study was performed by using a
strictly controlled temperature gradient incubator (T.G.I.). The
choriocarcinoma cells, clone GCH-nu-YS derived from an established
choriocarcinoma cell-line designated GCH-nu were placed into condition of
various temperatures (37.0-42.4 Celsius) for about 2 weeks and inhibitory
effect of the cellular proliferation and secretion of Human Chorionic
Gonadotropin (HCG) into media were chronologically investigated. When the
cells were cultured at 39.0-39.2 Celsius, the proliferation of YS clone
was nearly as same as that of the cells cultured at 37.0 Celsius. The
proliferation at 39.5-39.6 Celsius was slightly inhibited, whereas it was
inhibited markedly with increasing tendency when cultured over 39.9
Celsius. Above 40.4 Celsius the cellular proliferation was impossible,
thus resulting in destruction of the cells. These data suggest that
temperature upper-limit for proliferation (TLP) is approximately 40.1-40.2
Celsius and temperature upper-limit for viability (TLV) is approximately
40.4-40.6 Celsius. According to Okumura's classification for temperature
sensitivity, it is considered that the choriocarcinoma cells are resembled
to the characteristics of adenocarcinoma. HCG secretion was dramatically
affected by high temperatures, being suppressed until 1/24 of the control
at 39.0-39.2 Celsius at which temperature the cellular proliferation was
not so inhibited. At temperatures over 39.5 Celsius, HCG secretion was
decreased inversely elevating temperatures. And above 40.6 Celsius (over
TLV), HCG secretion was not recognized. From the results of this study, it
is believed that hyperthermic therapy will be a new treatment against
choriocarcinoma in the future.
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