| Publication number |
CLPUB00314 |
|---|
| Authors |
Yu H.-C., Zheng G.-L., Wang X.-Y., Li G.-X., Zhao K.-J. |
|---|
| Title |
Establishment of a cell line from embryos of Mythimna separata and its susceptibility to MSNPV. |
|---|
| Citation |
Virol. Sin. 18:31-34(2003) |
|---|
| Web pages |
https://en.cnki.com.cn/Article_en/CJFDTotal-ZBDX200301008.htm |
|---|
| Abstract |
A new cell line, designated NEAU-Ms-980312, was established from 24-hr-old
Mythimna separata eggs and was passaged 201 times. The cells were
heterogeneous in morphology with spherical and spindle-shaped cells. The
population doubling time of the cell line was 47.82h in 120h at 28 Celsius.
The cell line was highly susceptible to Mythimna separata nuclear
polyhedrosis virus (MsNPV). When the cells were inoculated at a
multiplicity of infection (m.o.i.) of 0.08TCID50/ cell with wild passage
MsNPV, the infection rates and occlusion bodies (OB) yield reached a value
of 62.10%, 33.59PIB/infected cell at 240h postinfection (pi), respectively.
|
|---|
| Cell lines |
CVCL_AV36; NEAU-Ms-980312 |
|---|
