| Publication number |
CLPUB00311 |
|---|
| Authors |
Patricio Cabane, Patricio Gac, Marcos Ramirez, Ricardo Rossi, Sofia Oviedo, Carmen Romero, Pablo Caviedes, Flavio Salazar-Onfray, Lorena Salazar, Mercedes Lopez, Leonor Moyano, Raul Caviedes; |
|---|
| Title |
Characterization of human parathyroid cell line (RCPTH) and tumorigenicity evaluation in NOD/SCID. |
|---|
| Citation |
Rev. Chil. Endocrinol. Diabetes 6:59-68(2013) |
|---|
| Web pages |
https://pesquisa.bvsalud.org/portal/resource/pt/lil-726576 |
|---|
| Abstract |
Background: Cell therapy could be an alternative for the treatment of
hypoparathyroidism. Therefore efforts have been made to establish a cell
line of parathyroid cells.
Aim: To establish a continuous functional and non-tumorigenic human
parathyroid cell line.
Material and methods: Nineteen tissue samples from 15 patients subjected
to parathyroidectomy due to primary or secondary hyperparathyroidism were
obtained. Functional, morphological and tumorigenic properties of the
obtained cells were analyzed.
Results: After two months of culture in conditions of immortalization,
cells had an exponential growth without experiencing senescence.
Therefore, more than 200 sub cultures have been performed. The cell line
was denominated RCPTH. Morphological characterization showed monolayer
growth with contact inhibition and a duplication time of 30 hours. On
light microscopy, pleomorphism and low number of mitoses were observed.
Cells accumulated glycogen, expressed calcium sensing receptor and had
positive PTH cytoplasmic clusters. The line secreted PTH initially but
subsequently, PTH production became undetectable. The cell line did not
have tumor or metastatic growth.
Conclusions: A parathyroid cell line has been established. The lack of PTH
production is a problem that will require the search for mechanisms to
activate it.
|
|---|
| Cell lines |
CVCL_9Y59; RCPTH |
|---|
