| Publication number |
CLPUB00304 |
|---|
| Authors |
Zhang H., Li X., Miao L., Wang H.-T., Zhang A.-J., Du H., Yang Q., Qin Q.-L. |
|---|
| Title |
Establishment of cell lines from the fat bodies of Spodoptera exigua larvae. |
|---|
| Citation |
Zhongguo Xi Bao Sheng Wu Xue Xue Bao 31:726-730(2009) |
|---|
| Web pages |
https://en.cnki.com.cn/Article_en/CJFDTotal-XBZZ200905025.htm |
|---|
| Abstract |
This study focused on how to initiate insect cell lines from primary
culture using the fat bodies of Spodoptera exigua larvae as culture tissue.
Factors influencing proliferation of new cells from the primary culture,
how to deal with subculture, as well as impact of microorganism pollution
or medium crystallization on the primary culture were investigated and
discussed. The results showed that the treatment that ensured the cultured
tissue fast adhere to the culture surface was very important for new cells
proliferating from the tissue. In the all adhesion cultures there were 62%
of the cultures observed cell proliferation. Those continuous
proliferating and further covering the whole culture surface were of 33%.
Finally, approximate 15% of the primary cultures were developed to
continuous cell lines. The average time from initial training to the
success of the first passage was 85 days. Pollution and crystallization of
the primary culture were important causations of failure.
|
|---|
| Cell lines |
CVCL_0A49; IOZCAS-Spex-II CVCL_0A51; IOZCAS-Spex-III CVCL_0A52; IOZCAS-Spex-IV CVCL_0A54; IOZCAS-Spex-VI CVCL_0A55; IOZCAS-Spex-VII |
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