| Authors |
Cencic A., Gradisnik L., Vaukner M., Filipi B., Rannou O., Chingwaru W., Maragkoudakis P., Tsakalidou E., Lefevre F. |
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| Abstract |
Up to-date, very few intestinal cell models exist to mimic the
interactions in the intestinal tract of animals and man. Among the
existing ones, human cancerogenic cell lines like Caco2 are widely used.
As cancerogenic, these cell lines are not appropriate model to perform
studies in normal, noncancerogenic intestinal environment. Therefore, we
developed several epithelial cell lines of animal small intestinal origin
and cells of immune system to enable studies of interactions between food
and water born viruses (rotavirus, TGEV and HEV) and the animal or human
host. A complete pig intestinal functional cell developed consists of:
small epithelial intestinal cells: CLAB -enterocytes obtained from the
adult pig, PSI -Pig Small Intestine (clones 13), characterized as
nondifferentiated intestinal epithelial cells from the adult pig, PoM -Pig
Monocytes/macrophages cell lines, PoMon -Pig Monocytes obtained from the
peripheral blood and dendritic cells (DCs) that were developed by an in
vitro differentiation system to obtain routinely porcine immature DCs from
purified blood monocytes. Among ruminants, calf small intestinal cell line
CIEB -Calf Small Intestinal cells B; was obtained from the 450 kg
Limousine calf, OSI -Ovine Small Intestinal cells; were obtained from the
sheep, and goat, GIE -Small Intestinal cell line was obtained from the
small intestine of the animal. Cell lines obtained were tested for
susceptibility to rotavirus, TGEV and HEV infection and propagation. A
substantial variety in the ability of each individual virus to infect and
propagate in developed cell lines was observed, indicating specificity in
the virus host interactions. Based on obtained results, we can conclude
that newly developed animal intestinal cell models can serve as excellent
experimental tools in studies of virus host interactions in the intestinal
tract of animals and human.
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