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Cellosaurus publication CLPUB00287

Publication number CLPUB00287
Authors Cencic A., Gradinik L., Vaukner M., Filipi B., Rannou O., Chingwaru W., Maragkoudakis P., Tsakalidou E., Lefevre F.
Title Intestinal cell models as alternative to experimental animals in research of food and water born viruses interactions with the host.
Citation Exp. Pathol. Health Sci. 1:57-57(2007)
Abstract Up to-date, very few intestinal cell models exist to mimic the interactions in the intestinal tract of animals and man. Among the existing ones, human cancerogenic cell lines like Caco2 are widely used. As cancerogenic, these cell lines are not appropriate model to perform studies in normal, noncancerogenic intestinal environment. Therefore, we developed several epithelial cell lines of animal small intestinal origin and cells of immune system to enable studies of interactions between food and water born viruses (rotavirus, TGEV and HEV) and the animal or human host. A complete pig intestinal functional cell developed consists of: small epithelial intestinal cells: CLAB -enterocytes obtained from the adult pig, PSI -Pig Small Intestine (clones 13), characterized as nondifferentiated intestinal epithelial cells from the adult pig, PoM -Pig Monocytes/macrophages cell lines, PoMon -Pig Monocytes obtained from the peripheral blood and dendritic cells (DCs) that were developed by an in vitro differentiation system to obtain routinely porcine immature DCs from purified blood monocytes. Among ruminants, calf small intestinal cell line CIEB -Calf Small Intestinal cells B; was obtained from the 450 kg Limousine calf, OSI -Ovine Small Intestinal cells; were obtained from the sheep, and goat, GIE -Small Intestinal cell line was obtained from the small intestine of the animal. Cell lines obtained were tested for susceptibility to rotavirus, TGEV and HEV infection and propagation. A substantial variety in the ability of each individual virus to infect and propagate in developed cell lines was observed, indicating specificity in the virus host interactions. Based on obtained results, we can conclude that newly developed animal intestinal cell models can serve as excellent experimental tools in studies of virus host interactions in the intestinal tract of animals and human.
Cell lines CVCL_6A77; CIEB
CVCL_6A79; PSI-1