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Cellosaurus publication CLPUB00285

Publication number CLPUB00285
Authors Tabata N., Katsuki T., Kumura K., Nakagawa N., Kondo K., Nagatomo H., Ninamishima Y.
Title Establishment and characterization of a rat hepatocyte line transformed by the SV40 T antigen gene.
Citation Artif. Organs Today 3:43-56(1993)
Abstract Hepatocytes isolated from an adult male Wistar rat were transfected with plasmid pMTIOD containing the early region of simian virus 40 (SV40) genome by using the electroporation method and a hepatocyte line, designated RTH33, has been established from the transformed cells. The presence of SV40 T antigen in the RTH33 cells showed that the cell line resulted from the introduction of SV40 genetic information. RTH33 cells were similar in morphology to the primary hepatocytes in monolayer culture. The cells multiplied vigorously in a serum-containing medium and, as is evident in the case of primary hepatocytes, the DNA synthesis was stimulated by insulin and the epidermal growth factor (EGF). RTH33 cells retained hepatocytic functions including albumin synthesis, urea synthesis, gluconeogenesis, ketogenesis and glucuronidation of bilirubin even after more than 100 passages over a period of 2 years. The cell line, RTH33, is a useful and stable cell source not only for biological studies of hepatocytes, but also in the development of bioartificial livers using cultured hepatocytes, which is a promising therapeutic method in the case of fulminant hepatic failure.
Cell lines CVCL_8753; RTH33