| Abstract |
MSL-G2 cells have previously been described as a highly heterogenous clone
with multiple hormone/neurotransmitter expression. MSL-G2 cells
preferentially express glucagon, cholecystokinin (CCK), and islet amyloid
polypeptide (IAPP), but display small populations of CGRP, insulin, and
somatostatin positive cells. Also the neurotransmitters serotonin and GABA
are expressed in the majority of MSL-G2 cells. MSL-G2 is known to change
its phenotypical composition when passaged in vivo and stable insulinomas
as well as glucagonomas has been established. We now report the
establishment of a highly stable somatostatinoma culture (MSL-G2-Tu6)
which has been maintained in continous culture for more than a year.
Almost 100% of the cells express high levels of immunoreactive
somatostatin. Few insulin, glucagon, CCK, and IAPP cells are still present.
Interestingly, a significant fraction of the cells expressed (3-endorphin
immunoreativity, implicating activation of the POMC gene. The derivation
of the somatostatinoma cell line MSL-G2-Tu6 further support the stemcell
nature of MSL cells. MSL-G2-Tu6 cells constitutes an ideal cell line in
which to study tissue-specific expression of the somatostatin gene.
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