| Abstract |
A human glioblastoma multiforme cell line has been established from a
glioma on the frontal lobe of a senior man. This cell line was designated
BT 325 and has been maintained in culture for over four years. The
morphologic features of cells are variable. Globose, bipolar, stellate and
multinuclear giant cells have been observed from the culture. Doubling
time calculated from the growth curve was approximately 36.5 hours. The
karyotype was shown hypo-tetraploid and had one marker chromosome in most
cells. Cell cycle phase and relative DNA content in cells were measured
from 9th to 57th passage by FCM and PLM. Cells inoculated into nude mice
subcutaneously grew into tumors similar to the original tumour
histologically. Microtubules and bundles of filaments can be observed by
electron microscope. Cells are positively stained by antiserum to GFAP.
Results of PTAH stain and Foot's stain proved these cells are malignant
glioma in no time. The rate of vitality of those frozen cells around 77%
showed the growth and character of the cell line was stable. It was
helpful in selection of anti glioma chemotherapy.
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