| Abstract |
Fibrosarcoma is a deadly disease in cats and is most often located at
classical vaccine injections sites. More rare forms of spontaneous
non-vaccination site (NSV) fibrosarcomas have been described and have been
found associated to genetic alterations.
Purpose of this study was to compare the efficacy of adenoviral gene
therapy in NVS fibrosarcoma. The NVS fibrosarcoma cell line (Cocca-6A) was
isolated from a spontaneous fibrosarcoma that occurred in a domestic
calico cat. Cocca-6A fibrosarcoma cells were morphologically and
cytogenetically characterized. The feline cells were karyotyped and their
chromosome number was counted using a Giemsa staining. Giemsa block
staining of metaphase spreads of the Cocca-6A cells demonstrated deletion
of one of the E1 chromosomes, where feline p53 maps. Adenoviral gene
therapy demonstrated a remarkable effect on the viability and growth of
the Cocca-6A cells following transduction with adenoviruses carrying
RB/p105, Ras-DN, Mda-7/IL-24 or their combination. Adenoviral gene
transfer was verified by western blot analysis. Flow cytometry assay and
annexin-V were used to study cell-cycle changes and cell death of
transduced cells.
Therapy for feline fibrosarcomas is often insufficient for long lasting
tumor eradication. The use of Ad.Ras-DN, Ad.Rb/p105 and Ad.Mda7/IL-24 has
been identified as a gene therapy for a possible future adjuvant treatment
in spontaneous feline fibrosarcoma. More gene therapy studies should be
conducted in order to understand if these viral vectors could be
applicable regardless the origin (spontaneous vs. vaccine induced) of
feline fibrosarcomas.
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