| Publication number |
CLPUB00236 |
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| Authors |
Yang Y.-X., Meng Y., Zhou L.-J., Li Y.-P., Zheng J.-L., Zhang W.-X., Lin J.-X., Zhang P. |
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| Title |
Establishment of a multidrug resistance cell line SO-Rb50/CBP of human retinoblastoma and expression analysis of drug resistance associated genes. |
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| Citation |
Zhonghua Lin Chuang Yi Shi Za Zhi 4:2167-2174(2010) |
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| Web pages |
http://216.17.69.49/KCMS/detail/detail.aspx?filename=ZLYD201011022&dbcode=CJFD&dbname=CJFD2010 |
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| Abstract |
Objective: To establish a carboplatin-resistance human retinoblastoma cell
line SO-Rb50/CBP and study its biological characteristics and the probable
drug-resistance mechanism.
Methods: First, SO-Rb50/CBP was induced by intermittently administration
of high-concentration of carboplatin (CBP). Then MTT assay was used to
detect drug sensitivity of the two cell lines. Then reverse transcripts
polymerase chain reaction (RT-PCR) was employed to detect the
transcription of MRP and GST-pi genes in cells of SO-Rb50 and SO-Rb50/CBP
respectively. Immunohistochemical technique and flow cytometry were used
to detect the proteins of MRP and GST-pi of the above cells.
Results: The drug-resistance index of SO-Rb50/CBP cell line to CBP was 15.7.
The population doubling time of SO-Rb50 and SO-Rb50/CBP were 44.32 h
and 50.9 h respectively. The drug-resistance indices of SO-Rb50/CBP to DDP,
VCR, MMC, VP-16 and 5-FU were 6.89, 5.68, 4.58, 2.28 and 1.88 respectively.
The positive rate of GST-pi and MRP in SO-Rb50/CBP cells were 96.19% and
90.21% respectively by immunocytochemistry, while in SO-Rb50 cells were
20.12% and 30.48% respectively. The mRNA level of MRP and GST-pi were higher
in SO-Rb50/CBP cells than that in SO-Rb50 cells.
Conclusions: The established carboplatin-resistance human retinoblastoma
cell line SO-Rb50/CBP showed resistance to several other drugs such as
MMC, DDP, VCR, VP-16. The drug-resistance mechanism of SO-Rb50/CBP cells
maybe due to high expression of MRP and GST-pi genes.
|
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| Cell lines |
CVCL_D543; SO-Rb50 CVCL_0U08; SO-Rb50/CBP |
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