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Cellosaurus publication CLPUB00233

Publication number CLPUB00233
Authors Lightbody J.
Title Establishment of differentiated clonal strains of glial brain cells in culture.
Citation Fed. Proc. 27:720-720(1968)
Abstract Brain tumors, composed primarily of differentiated astrocyte like cells, were induced in rats by weekly injections of 5 mg/kg N-nitrosomethylurea. Passage of the tumor cells through culture greatly enhanced their ability to be serially transplanted. Cultures were established by the method of alternate culture and animal passage. The single-cell plating technique was used to develop clonal strains from these cultures. The presence of a highly acidic protein, named S-100, found only in vertebrate brain has been reported. S-100 has been found serologically in one (C6) of the clonal strains and not in the other (C3). About 0.2% of the total soluble protein is S-100 when the cells are in stationary phase. Data pertaining to the kinetics of S-100 production will be presented. The C6 strain has been serially cultured for about one year without loss of its ability to produce the brain specific protein S-100. The cells have a near diploid chromosome number, and upon subcutaneous injection into rats produce tumors in approximately 3 weeks. In contrast, the C3 strain does not produce a tumor. Thus, serially propagated clonal strains of brain. cells are able to perform at least one organ specific function for prolonged periods in culture.
Cell lines CVCL_0194; C6