| Publication number |
CLPUB00223 |
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| Authors |
Kim H.-J., Lee W.-Y., Kim M.-J., Tark D.-S., Cho I.-S., Sohn H.-J., Lee Y.-H. |
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| Title |
Gene expression profile of a persistently chronic wasting disease (CWD) prion-infected RK13 cell line. |
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| Citation |
J. Prev. Vet. Med. 36:186-195(2012) |
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| Web pages |
http://jpvm.kr/journal/article.php?code=21393 |
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| Abstract |
Chronic wasting disease (CWD) is a neurodegenerative disorder in cervids
and a member of the transmissible spongiform encephalopathies (TSEs), also
known as prion diseases. We previously generated a persistently CWD prion
infected RK13 cell line (RKC1-11) using elk PrPC expressing cells
(elkRK13) that were generated with the lentiviral expression system. To
investigate the differentially expressed (DE) genes involved in prion
infection at the cellular level, we performed microarray analysis and
identified the DE genes between CWD-infected (RKC1-11) and non-infected
cells (elkRK13). Collectively, 88 genes were found to be differentially
expressed (42 genes upregulated > 2-fold; 46 genes downregulated <0.5-fold);
additionally, 10 up- and 8 downregulated genes agreed with the
results of the qRT-PCR. Among these genes, we chose 8 DE genes associated
with cell growth, signal transduction, transport, immune response and
apoptosis based on gene function analysis for further analysis. The
expression of the selected genes was further validated using an animal
model in normal-and CWD-infected TgElk mice showing clinical signs at 185
dpi. These identified DE genes in both the in vitro and in vivo model
could help in understanding the diagnosis and pathogenesis of prion
diseases.
|
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| Cell lines |
CVCL_0A22; elkRK13 CVCL_0A23; RKC1-11 |
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