| Abstract |
A new cell line was established from Trichoplusia ni fat body in Excell
400 medium supplemented with 5% FBS. The maximum cell density reached
2.2x10^6 cells mL and the population doubling time was 22.9 h for this cell
line. The cell line was highly sensitive to AcMNPV, and produced 86
occlusion bodies per cell. The expression levels for two recombinant
proteins, beta-galactosidase and alkaline phosphatase, in this cell line
were compared to that in IPLB-Sf-21 and BTI-5B1. The activities of beta-
galactosidase and alkaline phosphatase from the novel cell line were (225.5
+- 13.4) IU mL and (4.7 +- 0.61) IU mL respectively. The expression levels
from the fat body cells and from BTI-TN-5B1, which is known to express
recombinant proteins at the highest levels, were almost the same and the
former was a little bit better. The new cell line can efficiently support
the expression of foreign protein and is designated as HNU-Tn-FB1.
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