| Abstract |
The development of new cell lines from unusual and non-pest species may
provide important source for basic research and biotechnology application.
In this study, three new insect cell lines designated as QB-Ms1-8, QB-Ms2-2,
and QB-Ms2-7, respectively, were established from eggs of Manduca sexta.
These cells were subcultured approximately 50 passages in TNM-FH medium at
28 Celsius. The majority of cells of the lines generally are spindle-
shaped. The population doubling time of QB-Ms1-8, QB-Ms2-2 and QB-Ms2-7
cells was 51, 31 and 49 h, respectively. Although these lines were
insusceptible to Autographa californica multiple nuclear polyhedrosis
virus (AcMNPV) with the infection rate of 33%-40% at 96 h post infection,
QB-Ms2-2 cell line showed higher level of secreted SEAP production in
comparison with BTI-Tn5B1-4 cell line. In this study, a SEAP high
expression type cell line, QB-M2-2 was isolated from the three new cell
lines established from M. sexta, which may provide a source for searching
novel clones with higher production of recombinant proteins.
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