| Publication number |
CLPUB00216 |
|---|
| Authors |
E. Clinton Lawrence, George W. Daneker Jr., Phyllis A. Waite-Rees, Serena A. Lund, Francisco J. Candal, Daniel C. Bosse, R. Kuno, Kirk Randy Kanter, Edwin W. Ades; |
|---|
| Title |
Establishment of immortalized human lung microvascular endothelial cell lines. |
|---|
| Citation |
Chest 108 Suppl. 3:126S-126S(1995) |
|---|
| Abstract |
Introduction: Human lung microvascular endothelial cells (HuLEC) are the
primary targets for acute allograft rejection.
Methods: HuLEC were isolated independently (GWD and ECL), then
immortalized as previously described (Ades, et al. J. Invest. Dermatol.
99:683-90, 1992) The cell lines, CDC.EU./HuLEC-1 and 2, were characterized
for endogenous expression of various molecules using flow cytometry.
Results: Both cell lines constitutively expressed HLA class I but not
class II alloantigens. Features characteristic of endothelial cells, i.e.
staining for low density lipoprotein and von Willebrand's factor were
present in both cell lines.
Conclusions: HuLEC have been isolated and immortalized, providing tools
for further study of the mechanisms underlying acute allograft rejection
following lung transplantation.
|
|---|
| Cell lines |
CVCL_0A09; HuLEC-1 CVCL_0A10; HuLEC-2 |
|---|
