| Abstract |
A cell strain (designated KIZ-81A) derived from lung tissue of a young
male tufted deer (E. cephalophus) is presented. The animal was caught in
southern China in 1981. The KIZ-81A cells were fibroblasts in morphology.
The primary cells died at passage 67 after 13 mo. in vitro, while KIZ-81A
cells, which had been frozen since passage 3 and kept in liquid nitrogen
for 132 days before thawing, attained only 64 passages. Growth rates of
KIZ-81A cells were determined at passage 7, 11, 30. Comparison was made at
generation 11 of primary cells and cells once frozen. After a lag phase,
all cultures entered the logarithmic growth phase and then the stationary
phase again. The range of cell cycle time, determined by
BUdR[bromodeoxyuridine]-Giemsa method for detection of sister chromatid
exchanges, was from 24-36 h. Karyotype analysis revealed the chromosome
number and construction in KIZ-81A cells (without being frozen and stored)
were more stable than those of the cells once frozen in liquid nitrogen.
In the former, the obvious increase of chromosome number and changes in
construction started from the 51st generation and in the latter from the
30th generation. Cells with chromosome number from 49-52 greatly increased.
Effects of some experimental factors, including temperature and pH
changes, on cell growth and division are also analyzed and discussed.
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