| Publication number |
CLPUB00192 |
|---|
| Authors |
Zhao Y.-G., Lan M.-Y., Meng Y.-C. |
|---|
| Title |
Establishment and characterization of a mosquito cell line from Culex pipiens pallens. |
|---|
| Citation |
Chin. J. Schist. Cont. 4:281-284(1992) |
|---|
| Abstract |
A mosquito cell line Cpp512 was established from newly hatched. 1st instar
larvae of Culex pipiens pallens. The surface of mosquito eggs were
sterilized, the larvae were cut into pieces and placed in modified TC199
containing 15% FBS. It was about 2 weeks for the cell to form a monolayer
from the larvae fragments. The subculture was made by 1 t 3-6 split every
week with rubber policeman or pipete. And now 53 subculture have been
passed. The cell were mainly in spindal like shape. The population
doubling time was 21 hours and cell cycle time was 18 hours. Observation
with TEM showed no contamination of virus or micoplasma. The chromosome
examination at different passage level showed the Cpp512 cell line were
mainly diploid, but polyploid also existed.
|
|---|
| Cell lines |
CVCL_Z447; Cpp-512 |
|---|
