Cellosaurus logo
expasy logo

Cellosaurus publication CLPUB00190

Publication number CLPUB00190
Authors Zao Y.-G., Yu C.-X., Lu W.-X., Yin X.-R., Lan M.-Y.
Title Application of random amplified polymorphisms DNA polymerase chain reaction (RAPD-PCR) method in the identification of mosquito cells.
Citation Zhongguo Mei Jie Sheng Wu Xue Ji Kong Zhi Za Zhi 4:401-403(1993)
Web pages http://www.bmsw.net.cn/CN/Y1993/V4/I6/401
Abstract Primer Pm was screened by RAPD-PCR. Four separate DNA maps were obtained through amplifying the genomic DNA of the mosquito cells (Anso-242, Cpp-512, Ana-104, C6/36) with the primer Pm. The molecular weights (kb) of the DNA bands of each map: Anso-242 0.50, 0.88, 1.02, 1.13, 1.22, 1.5, 2.1, 2.4, 2.5; Cpp-512 0.5, 0.7, 0.8, 0.88, 1.02, 1.22; Ana-104 0.64, 0.98, 1.2, 1.28, 1.4, 1.53, 1.8, 2.3, 2.6, 3.3; C6/36 0.66, 0.8, 0.94, 1.4, 1.42, 1.5, 1.52, 2.9. The test result showed that RAPD-PCR method is simple and feasible for biological classification. The results are stable and reproducible. The Primer Pm may satisfy the needs for the identification of the four mosquito cells.
Cell lines CVCL_Z534; Ana-104
CVCL_Z536; Anso-242
CVCL_Z447; Cpp-512