Abstract |
Primer Pm was screened by RAPD-PCR. Four separate DNA maps were obtained
through amplifying the genomic DNA of the mosquito cells (Anso-242,
Cpp-512, Ana-104, C6/36) with the primer Pm. The molecular weights (kb) of
the DNA bands of each map: Anso-242 0.50, 0.88, 1.02, 1.13, 1.22, 1.5, 2.1,
2.4, 2.5; Cpp-512 0.5, 0.7, 0.8, 0.88, 1.02, 1.22; Ana-104 0.64, 0.98, 1.2,
1.28, 1.4, 1.53, 1.8, 2.3, 2.6, 3.3; C6/36 0.66, 0.8, 0.94, 1.4, 1.42, 1.5,
1.52, 2.9. The test result showed that RAPD-PCR method is simple and
feasible for biological classification. The results are stable and
reproducible. The Primer Pm may satisfy the needs for the identification
of the four mosquito cells.
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