| Abstract |
A cell line from Anopheles anthrophagus, Ana 104, is established from
1st instar larvae. The medium is TC199, supplemented with 0.4% lactalbumin
hydrolysate (LH), 0.03% glutamine, 10-20% FBS and adequate amount of
penicillin and streptomycin as well as non essential amino acids. The
fragment of the larvae remained active for 2.5 months and continuously
produced cells. After 7-10 days of subculture, the cells formed a
monolayer which could be maintained for more than 22 days. The cell line
is on its 27 passage level now. The cell is adapt to the medium L-15,
DMEM etc. From Oth passage on some cells were stored in liquid nitrogen
and successfully resuscitated. Ana 104 mainly contains epithelial type
cells, spindal like cells also exist. The chromosome number is 2n=6, The
observation with electron microscope showed no contamination of virus or
mycoplasma. The dynamic study of amino acids requirement indicated that
the cells consumed relatively large amount of Glu, Tyr, Orn, Arg, Cys,
etc., but increased the concentration of Asp in the medium. The activity
of carboxylesterase and cholinesterase were detected. Esterase zymograms
from polyacrylamide gel electrophoresis revealed 3 bands. The Anopheles
anthropophagus is addicted to human blood, it is important vector in
China. Ana 104 was the 4th cell line established from Anopholine mosquito,
The other three were established from An. gambia, An. stephensi, An.
sinensis. The cell line Ana 104 would be helpful in the study of virology,
parasitology, cell and molecular biology and its management.
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