Cellosaurus logo
expasy logo

Cellosaurus publication CLPUB00185

Publication number CLPUB00185
Authors Kakpakov V.T., Gvozdev V.A., Platova T.P., Polukarova L.G.
Title In vitro establishment of embryonic cell lines of Drosophila melanogaster.
Citation Genetika 5:67-75(1969)
Abstract In vitro establishment of embryonic cell lines of Drosophila melanogaster is described. The enriched C15 medium was used for cell culture. The population-doubling time was 24 h; it did not decrease after the 50 passages. Two sublines with female karyotypes were obtained: 1) diploid subline 67j25D, containing 85-90 percent of diploids; 2) aneuploid subline 67j25A, containing 60-70 percent of aneuploids. After the prolonged cultivation of aneuploid subline a shift of the modal class of aneuploids was marked. For the growth of sublines the presence of bovine fetal serum containing non-dialysed thermostable factor is essential. The addition of pupae extract containing the thermolabile non-dialysed factor(s), particularly at the early passages, is necessary for the active growth of cells.
Cell lines CVCL_WY64; 67j25A
CVCL_Z425; 67j25D