Abstract |
Recently, continuous cell lines of insects increased in number by
improvement of culture media and procedures. According to Hink, it reached
more than 130 from more than 60 species of invertebrates. In the family of
Tortricidae, eight continuous cell lines from three species have been
established by Hink and Ellis, Sohi, Mahr and Miltenburger (unpublished),
and Peter (unpublished). Eight new cell lines from six species have been
added to the list. These six species are Adoxophyes orana fasciata,
Adoxophyes sp., Pandemis heparana, Archippus breviplicanus, Hoshinoa
longicellana. and Homona magnanima. They are serious pests for fruit
growing and tea cultivation in Japan. With some of them, microbiological
control using insect viruses has been demonstrated; however, the
fundamental properties of these viruses are not so clear for want of
established cell lines that are capable of replicating these viruses. In
this paper, the method used for establishing several characteristics of
eight new continuous cell lines including susceptibility to insect
pathogenic viruses are described.
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