| Abstract |
BCIRL-SO3-HNU1 and BCIRL-SO4-HNU4 were two new cell lines derived from the
ovarioles of the yellow-striped armyworm or cotton cutworm, Spodoptera
ornithogalle. The ovarioles of a newly emerged female moth were sectioned
and the cells treated with trypsin and cultured in Falcon TC-25cm2 flasks
each with 5 ml of TC-199-MK, medium. The most predominant characteristics
of these cell lines during development is that the rapid growth was not
only observed in the primary cultures, but their quick multiplication can
also be seen in the successive passages. The durations of SO3 and SO4 from
the primary culture to the first passage lasted only 28 and 29 days
respectively. After the fourth passage, a 1:2 split ratio was made three
times a week, while the duration of each of the first 4 passages was 3-5
days. Then, after a succession of 100 passages, a change of split ratio of
1:10 was made and the passage intervals were once a week. Both the cells
of SO3 and SO4 were loosely attached to the bottom of the flasks. The
subculture could be easily performed just by agitation or flushing medium
onto the cells without any trypsin treatment. The cells were mostly
rounded, with some elongated and spindled forms. The SO4 cells seemed to
be a little bit larger than those of SO3. At 28 Celsius during 120 hrs,
the cell doubling time for SO3 was 47 +- 3.2 hrs, while SO4 at 28 Celsius
during 72 hrs being 54 +- 5.07 hrs. The maximum cell densities of SO3 and
SO4 attained 1. 36x10^6/ml and 1.28x10^6/ml respectively. The chromosomes
were typical of Lepidoptera, they appeared to be blunt, short and
numerous, but variable in number. The results of the preliminary
experiments have shown that these two cell lines seemed to be neither
susceptible to the M-NPV of Spodoptera ornithogalle nor to that of the
Heliothis armigera.
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