| Abstract |
The analysis of gene expression in human lymphocytes by two-dimensional
polyacrylamide gel electrophoresis (2-D) has been the subject of many
papers. The identification of protein sets that characterize both the
normal and abnormal phenotypes of different subpopulations has been
documented. We have also shown that regulation studies performed by the
selective introduction of a variety of biological response modifiers into
the labeling cultures can be carefully and reproducibly analyzed by 2-D
gels and the set or sets of proteins under their control can be defined.
The work done over the past few years is largely responsible for our
current understanding of human lymphocyte 2-D patterns. As a continuation
of my studies of gene expression in normal and abnormal human lymphocytes,
I have analyzed the human T-cell leukemia virus (HTLV)-infected cell line,
HUT-102, by high-resolution two dimensional gel electrophoresis.
Comparisons of this pattern with HTLV-negative cell lines such as Jurkat,
GM-607, and HL-60 were made in order to preliminarily identify a set of
protein-gene-products whose expression is either related to viral proteins
in the cell or viral induction of host cellular proteins. During this
study, I also attempted to establish cell lines from Belgian patients with
T-cell malignancies and have been successful in obtaining several
different lines. This paper will mainly concern the cell line, WE17/10,
which was the first to be established in my laboratory and has been the
most thoroughly characterized at the present time. It is interesting to
note that most incidences of HTLV-related leukemia in Europe have been
associated with individuals who immigrated from the endemic regions of the
world or native Europeans who travelled to those areas.
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