| Abstract |
Unusual cell lines derived from brain metastases of two patients have been
studied in detail. COLO 338, from a 44-year old male, was established
11/77 and COLO 508, COLO 509, COLO 510 and COLO 511, from a 71-year old
male, were established 9/80. All cell lines have been maintained in
continuous culture in RPMI 1640 or GEM 1717 media supplemented with 10%
v/v fetal bovine serum. Excellent growth of the cell lines was achieved in
serum-free media supplemented with 1.2% v/v hetastarch (HESPANL), a plasma
extender. Cell lines from both patients grew as epithelioid adherent round
or polygonal cells. Ultrastructural details were similar for COLO 338 and
COLO 508 with the exception of numerous neurosecretory-like granules in
COLO 338. Biochemical characterization of the lines included assay of
peptide hormones, carcinoembryonic antigen secretion, estrogen,
progesterone, glucorcorticoid and androgen receptor proteins, and of
allozyme profiles. Cytogenetic analysis of COLO 508 revealed a bimodal
karyotype (55,58) and marker chromosomes including a ring chromosome and a
dicentric. COLO 338 was bimodal (60,62) and up to 40 different marker
chromosomes were observed in various cells. These cell lines are available
to other investigators.
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