| Abstract |
Recently we established three fibroblast cell lines from ear skin explants
of normal healthy dairy goats, of Kiko and Saanen breed (In Vitro Cell. Dev.
Biol. Animal; doi:10.1007/s11626-010-9373-4). The cell lines revealed a
viability rate of 96.2%, displayed a typical 'S' shaped growth curve with
a population doubling time of 25 hours, and were free from microbial,
fungal and mycoplasma contamination. We further characterized these cell
lines and, in this communication we show the cytogenetic analysis and the
genetic transfection of GSF289 cells. The GSF289 cell line which
originated from Saanen breed of goats was successfully transfected with
pcDNA3.1/NT-GFP plasmid vector containing green fluorescent protein (GFP)
gene under human cytomegalovirus (CMV) promoter. The efficiency of
transfection, as measured by flow cytometry, was 14.5% after 4 days of
culture. The cytogenetic analysis performed on 29 G-banded metaphase cells
revealed that the cell line has a normal male goat karyotype consisting of
58 autosomes and two XY sex chromosomes. These results suggest that GSF289
cell line with a normal karyotype, having a high rate of proliferation,
and its ability to be easily transfected with plasmid DNA vectors is an
additional tool to study molecular mechanisms that regulate fibroblast
function as well as genetic manipulation of small ruminants.
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