| Publication number |
CLPUB00130 |
|---|
| Authors |
Tasuku Watanabe, Tadaaki Moritomo; |
|---|
| Title |
Establishment and properties of a cell line derived from salmonid fish liver. |
|---|
| Citation |
(In book chapter) Invertebrate and fish tissue culture; Kuroda, Yukiaki & Kurstak, Edouard & Maramorosch, Karl (eds.); pp.199-202; Japan Sci. Soc.; Tokyo; Japan (1988) |
|---|
| Web pages |
https://www.springer.com/de/book/9783642736285 |
|---|
| Abstract |
The primary culture of embryonal liver cells was performed using the
embryonated eggs of Yamame Oncorhynchus masou. The cells, in 5 flasks of
22 attempts cultivated in TC199 with 10% FBS at 20 Celsius, consist of
epithelial cells with a round nucleus and abundant granular
intracytoplasmic organelles. One of them, designated YEL-13, grew in the
manner of contact-inhibited monolayer until the formation of confluent
cell sheets. The cells have been subcultured 90 times. YEL-13 cells were
found to produce and release a protein which had the antigenicity similar
to one of Yamame serum protein by fluorescent antibody method and
immunoelectrophoresis.
|
|---|
| Cell lines |
CVCL_R989; YEL-13 |
|---|
