| Publication number |
CLPUB00124 |
|---|
| Authors |
Suyama I., Etoh H. |
|---|
| Title |
Establishment of a cell line from Umbra limi (Umbridae; Pisces). |
|---|
| Citation |
(In) Invertebrate and fish tissue culture; Kuroda Y., Kurstak E., Maramorosch K. (eds.); pp.270-273; Japan Sci. Soc.; Tokyo (1988) |
|---|
| Web pages |
https://www.springer.com/de/book/9783642736285 |
|---|
| Abstract |
A fibroblast-like cell line (designated ULF-23) was established from the
fins of Umbra limi. The cell doubling time was estimated to be 72 hours
from the growth curve. It did not form colonies at all. The modal
chromosome number shifted to 23 after 50 passages, but it still kept
almost all other karyological characters. ULF-23 cells were irradiated
with X-rays for chromosome aberration analysis or treated with MNNG for
sister chromatid exchange (SCE) analysis. Both yields were lower than in
cultured mammalian cells, but their usefulness in in vitro fish
cytogenetic studies was confirmed.
|
|---|
| Cell lines |
CVCL_5641; ULF-23 |
|---|
