| Abstract |
A new cell line TY-ES was established from Ewing's sarcoma derived from a
14-years-old Japanese boy, and its characteristics were analyzed. This
cell line was found to be oncogenic in severe combined immunodeficiency
(SCID) mice. When incubated in a standard culture dish, this cell line
(TY-ES) grew in suspension, but in the presence of type I collagen and
gelatin, the cells grew and adhered to the dish wall. The doubling time of
the cells was 40 hours. When stained immunohistochemically, TY-ES cells
reacted positively with antibody to MIC2 (Ewing's sarcoma specific
antibody) and antibody to vimentin. They also showed partially positive
reactions to the stains for NFP and NSE. It was previously reported that
treatment with dibutyryl cyclic adenosine monophosphate (db-cAMP) induced
neural differentiation of Ewing's sarcoma cells. In the present study, we
examined the effects of db-cAMP treatment on the characteristics of TY-ES
and three other Ewing's sarcoma cell lines (SCCH-196, RD-ES and SK-ES).
The growth of all 4 cell lines was suppressed by db-cAMP treatment in a
dose-dependent manner. Tumor formation following transplantation of
db-cAMP-treated TY-ES or RD-ES cells into SCID mice was also significantly
suppressed. The growth of db-cAMP treated SK-ES or SCCH-196 cells in these
mice did not show significant suppression. Chemoinvasion, examined as an
indicator of tumor invasive potential, was reduced by db-cAMP treatment in
3 of the 4 cell lines excluding SK-ES.
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