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Cellosaurus publication CLPUB00092

Publication number CLPUB00092
Authors Etoh H., Suyama I., Hyodo-Taguchi Y., Matsudaira H.
Title Establishment and characteristics of various cell lines from Medaka (Teleostei).
Citation (In) Invertebrate and fish tissue culture; Kuroda Y., Kurstak E., Maramorosch K. (eds.); pp.266-269; Japan Sci. Soc.; Tokyo (1988)
Web pages https://www.springer.com/de/book/9783642736285
Abstract Four permanent cell lines, OLME-104 from melanoma of HB32C medaka, OLHE-131 from hepatoma of HO4C, OLF-136 from the normal fins of HB32C and OLF-124 from those of outbred medaka of orange-red variety, were established. From their growth curves, doubling times were estimated to be 43 h for OLF-136, 77 h for OLME-104 and 67 h for OLHE-131. Nearly diploid amounts of DNA for OLF-136 and OLME-104, but nearly tetraploid for OLHE-131 cells were observed on cytofluorometrical analysis and confirmed on metaphase preparations. The cultured cells were injected ip into respective host fish. No nodule was observed in HB32C medaka injected with OLF-136 cells. The fish injected with OLME-104 and with OLHE-131 cells died within a month due to tumor growth. The OLME-104 cells were transplantable to HB32C medaka but not to HO4C and outbred medaka. The OLHE-131 cells were transplantable only to HO4C. The tumor cells have maintained their malignant characters for many generations in in vitro culture. It seems from UV-irradiation experiments that these cell lines from medaka, as well as a cell line from goldfish, are more sensitive to UV-radiation compared to the cultured mammalian cells.
Cell lines CVCL_R980; OLF-124
CVCL_3766; OLF-136
CVCL_4873; OLHE-131
CVCL_3767; OLME-104