Cellosaurus publication CLPUB00087

Publication number	CLPUB00087
Authors	Inomata M., Sasaki M.S., Hirota T., Itabashi M., Tsumuraga M., Kaneko A., Hoshi A., Koyama Y., Saijo N.
Title	Establishment of a human retinoblastoma cell line: NCC-RbC-39.
Citation	Cancer J. 4:18-23(1991)
Abstract	A new human retinoblastoma cell line (NCC-RbC-39) was established from a tumor sample of a 3-year-old boy with a unilateral retinoblastoma. The components of the medium used to establish the retinoblastoma cell line were the same as used previously by us to grow colonies of cultured retinoblastoma cells. This medium was used with 12 consecutive fresh specimens to test its ability to support the growth of primary retinoblastoma cells. One specimen (NCC-RbC-39) from the 12 grew in the medium for more than a year. Several biological characteristics of the cells of the established NCC-RbC-39 line were compared with those of the cells from the original tumor. 1) The tumors produced in nude mice injected with NCC-RbC-39 cells showed a histopathological pattern similar to that of the original tumor. 2) The cells from both the original tumor and the established line showed a sensitivity to L-phenylalanine mustard in a human tumor clonogenic assay but loss of sensitivity to mitomycin C and adriamycin during the process of establishment. 3) Karyotypes of both the established cell line and the original tumor were diploid (46,XY) involving a common +1q chromosomal aberration with the established line having a few additional aberrations. This cell line appeared to conserve most of the original biological properties and may be useful for biological as well as drug sensitivity studies of retinoblastoma.
Cell lines	CVCL_3047; NCC-RbC-39