| Abstract |
CD36 antigen is a 90 kDa molecule expressed on monocytes, platelets,
hemopoietic progenitor cells, endothelial cells and some neoplastic cells.
It represents the receptor for thrombospondin, a 450 kDa glycoprotein
present in the bone marrow microenviroment. We isolated a new human cell
line (GM-490) from the marrow of a patient with acute lymphoblastic
leukemia. Morphologically, GM-490 has characteristics of fibroblastoid
cells and grows spontaneously in RPMI-10%FCS. Cytogenetic analysis
revealed a 56XY kariotype. Cytofluorimetric DNA analysis shoWed a high
number of cell in S phase (22,5%). Immunological phenotype was
characterized by high level expression of CD29, CD71 and CD36. All
erythroid, lymphoid, myeloid and megakaryocytic hematological markers were
negative. This cell line represents a useful model to study the
interaction between CD36 antigen and its physiological ligand. In addition
it may be useful to analyse the relationship between stromal cells,
extracellular matrix and hemopoietic cell precursors.
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