| Publication number |
CLPUB00041 |
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| Authors |
Nyla A. Heerema, Catherine G. Palmer, William Harvey, Jan Jansen, Edward F. Srour, Umnarj Paeratakul, D. Fisher, Milton W. Taylor; |
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| Title |
Telomere association in a hairy cell leukemia line (GASH). |
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| Citation |
Am. J. Hum. Genet. 41 Suppl.3:A122.359-A122.359(1987) |
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| Web pages |
https://pmc.ncbi.nlm.nih.gov/articles/PMC1684237/https://eurekamag.com/research/029/283/029283998.php |
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| Abstract |
Gash, a cell line established from a 34 year old male with hairy cell
leukemia has been confirmed as a hairy cell leukemia line by electron
microscopy, surface and cytoplasmic markers and positive TRAP (tartrate
resistant acid phosphatase) stain. The cell line involves rather mature
B-cells. Cytogenetic analysis showed 79% of the cells to be
46,XY,del(6)(p23), 13% to be pseudotetraploid with an exact duplication of
the pseudodiploid cells, and 8% to have random abnormalities in addition
to the deleted 6p. Twenty-seven percent of the pseudodiploid and 50% of
the pseudotetraploid metaphases had telomere association, a tight end-to-
end association of chromosomes, with one to three per metaphase. The
chromosomes involved appeared to be random with most chromosomes
represented. All but one association involved non-homologous chromosomes;
the only homologous telomeres associated were two 17p's in a tetraploid
cell. That these were in some cases more than transient contacts of
chromosomes was evidenced by the presence of rare cells (8%) with
translocations, fragments and terminal deletions--such abnormalities as
might result from breakage of a dicentric during division. Telomere
association has been reported previously in preparations from four
malignancies; two B-cell ALL, one T-cell ALL and one malignant fibrous
histiocytoma. It has also been reported in senescent fibroblasts, SV40
transformed cells and rare syndromes. The high frequency of telomere
association in this hairy cell leukemia line offers a unique opportunity
to study telomere association.
|
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| Cell lines |
CVCL_M709; GASH |
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