| Publication number |
CLPUB00038 |
|---|
| Authors |
Keating A., Martin P.J., Bernstein I.D., Papayannopoulou T., Raskind W., Singer J.W. |
|---|
| Title |
EM-2 and EM-3: two new Ph1+ myeloid cell lines. |
|---|
| Citation |
(In) Normal and neoplastic hematopoiesis, UCLA symposia on molecular and cellular biology, New Series Vol. 9; Golde D.W., Marks P.M. (eds.); pp.513-520; Alan R. Liss; New York (1983) |
|---|
| Abstract |
Two new cell lines, EM-2 and EM-3, were established from a patient who
relapsed with chronic myelogenous leukemia (CML) after marrow
transplantation. Both lines have been in continuous culture for one year,
are heteroploid with multiple copies of the Ph1 chromosome, and are of
host origin. Both lines expressed an increased frequency of NBT dye-
reducing cells after incubation with retinoic acid (RA). The lines were
also examined for the expression of myeloid determinants recognized by a
panel of monoclonal antibodies before and after incubation with RA or
phorbol-ester (TPA). Similar studies were conducted with BB-2 monoclonal
antibody recognizing a B cell blast associated antigen. We found that EM-2
and EM-3 have myeloid features and that both lines could be induced to
undergo further myeloid differentiation with TPA and/or RA. Incubation of
both lines with TPA increased the expression of the B cell blast
associated antigen.
|
|---|
| Cell lines |
CVCL_1196; EM-2 CVCL_2033; EM-3 |
|---|
