| Abstract |
An epithelial, cell line designated DND-10B was established from
peritoneal mesothelioma tissue of a 66 year old insulation trade worker
(blood type 0+) exposed to asbestos. The original specimen, obtained in
1975, was cut into <1mm3 pieces, suspended in RPMI 1640 medium with 20%
fetal calf serum, penicillin, streptomycin, and placed in plastic flasks.
The cells grow as a monolayer with a doubling time of 48 hr; they have
been subcultured more than 30 times. When subcultured the cells grow by
extending pseudopods. When densely propagated some grow in sheets and
others stack in layers; individual cells are cuboidal or polygonal in
shape. They contain abundant cytoplasm, with one or two nuclei and
prominent nucleoli. Nuclear chromatin is relatively fine. "Giant forms"
constitute less than 1% of the population. On electron microscopy cells
are equipped with microvilli, tight junction-like structures,
tonofilaments, and a well developed Golgi apparatus. These ultrastructural
findings are similar to those of the original mesothelioma cells. Large
numbers of lipid (or lipo-proteinaceous) granules are present in the
established cells. Karyotypically, the cultured cells are hypotetraploid
(modal number of 81) with several marker chromosomes. The availability of
an established cell line representative of a cancer which has a striking
association with specific environmental exposure is of importance for
multiple areas of research.
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