| Abstract |
Cultured cells obtained from a biopsy specimen of stage IV human carcinoma
of the cervix, designated as CC-VGH-7-T, have been maintained in
continuous culture since September 8, 1979. These cells grew without
interruption for about 3 months and then subcultivated over 25 times up to
April 30, 1980. Various passages of these cells were frozen in liquid
nitrogen and they could be restarted in tissue culture. They exhibit
stable growth with doubling time of 34 hours and are free of mycoplasma
contamination. These cells appear epithelial in shape, showing no visible
contact region and multilayering growth. Some cells are multinucleated,
some have multiple nucleoli, and all have heavily vacuolated cytoplasm
with decreased cytoplasm-nucleus ratio. The karyologic study indicated
that the chromosome number of CC-VGH-7-T cells ranged from 33 to 86 with
the mode of 42, which was a typical neoplastic feature. The tumorigenicity
of CC-VGH-7-T is now tested in athymic nude mice. Two out of three athymic
mice which were inoculated with cultured cells developed tumors in 4 weeks.
Interestingly, these cells do not form colony by soft agar test.
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