| Publication number |
CLPUB00006 |
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| Authors |
Wang Z.-J., Li M., Zhao W.-C., Zhao G.-Q., Dong Z.-M. |
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| Title |
Establishment and characterization of lung adenocarcinoma cell lines with multidrug resistance. |
|---|
| Citation |
Life Sci. J. 4:13-16(2007) |
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| Web pages |
http://www.lifesciencesite.com/lsj/life0401/life-0401-03.pdf |
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| Abstract |
Objective: Many discoveries of multi drug resistance (MDR) have resulted
from studies with drug-resistant tumor cell lines as their models. Till
now, there has been no report on the detailed characterization of such a
cell line from lung adenocarcinoma (LA). By long-term exposure of an
established LA cell (A549 cell) to increased concentrations of paclitaxel,
we established a series of subcultures that were considerably more
resistant to this drug.
Methods: Paclitaxel-resistant sublines (A549/TXL) were established in
vitro by exposing to stepwise increased concentrations of the drug in a
cell culture medium. Biological morphology was analyzed by morphometry and
flow cytometry. The chemoresistance indexes of cells were measured by
methyl tetrazolium assay. Evaluation of growth, in vitro drug sensitivity,
and a pharmacokinetic study were performed.
Results: Compared with parent cells, the resistant sublines were smaller
and mixed with giant cells in different sizes and with different numbers
of nuclei. The resistant cells, A549/TXL20 were 19.3 times more resistant
to paclitaxel and 67.4 times more resistant to cisplatin than the parent
cells. The resistant cells also demonstrated cross-resistance to
mitomycin, vinblastine, hydroxycamptothecine, and 5-fluouracil (5-FU).
Compared with the A549 cell line, an unreasonably higher level of drug-
resistance and lower drug concentration was detected in A549/TXL20 cells
after exposure to the drug in the culture medium.
Conclusions: The paclitaxel-induced MDR sublines may be used as an
experimental system for the search of a means to overcome drug resistance
and elucidate possible mechanisms of acquired MDR involved in human lung
adenocarcinoma.
|
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| Cell lines |
CVCL_W192; A549/TXL5 CVCL_W190; A549/TXL10 CVCL_W191; A549/TXL20 |
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