Cellosaurus cell line SEES3-3L (CVCL

Cellosaurus SEES3-3L (CVCL_ZJ90)

Cross-references
Cell line name	SEES3-3L
Synonyms	3L
Accession	CVCL_ZJ90
Resource Identification Initiative	To cite this cell line use: SEES3-3L (RRID:CVCL_ZJ90)
Comments	Characteristics: Transfected with a pG-flox-CAGrtTA3G-IRES-Hyg-pA construct that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16. Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_1Q91 (SEES3)
Sex of cell	Male
Age at sampling	Blastocyst stage
Category	Embryonic stem cell
Publications	PubMed=32433964; DOI=10.1016/j.celrep.2020.107655 Yuhki Nakatake, Shigeru B.H. Ko, Alexei A. Sharov, Shunichi Wakabayashi, Miyako Murakami, Miki Sakota, Nana Chikazawa-Nohtomi, Chiaki Ookura, Saeko Sato, Noriko Ito, Madoka Ishikawa-Hirayama ...Show all 27 authors... , Siu Shan Mak, Lars Martin Jakt, Tomoo Ueno, Ken Hiratsuka, Misako Matsushita, Sravan K. Goparaju, Tomohiko Akiyama, Kei-ichiro Ishiguro, Mayumi Oda, Norio Gouda, Akihiro Umezawa, Hidenori Akutsu, Kunihiro Nishimura, Ryo Matoba, Osamu Ohara, Minoru S.H. Ko; Show fewer authors Generation and profiling of 2,135 human ESC lines for the systematic analyses of cell states perturbed by inducing single transcription factors. Cell Rep. 31:107655.1-107655.23(2020)
Encyclopedic resources	Wikidata; Q98132439
Entry history
Entry creation	02-Jul-2020
Last entry update	14-Aug-2025
Version number	8