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Cellosaurus ISE6 (CVCL_Z170)

[Text version]
Cell line name ISE6
Accession CVCL_Z170
Resource Identification Initiative To cite this cell line use: ISE6 (RRID:CVCL_Z170)
Comments Group: Patented cell line.
Group: Tick cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-11974.
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Omics: Metabolomics.
Omics: Proteomics.
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Ixodes scapularis (Black-legged tick) (Deer tick) (NCBI Taxonomy: 6945)
Sex of cell Sex unspecified
Age at sampling Embryo
Category Spontaneously immortalized cell line
Web pages Info; DSMZ; -; https://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/TickCellCultureMethods-4.pdf
Info; VectorBase; -; https://vectorbase.org/vectorbase/app/record/dataset/DS_0941d3180e
Info; VectorBase; -; https://vectorbase.org/vectorbase/app/record/dataset/DS_20cfb4a364
Provider; TCB; -; https://www.liverpool.ac.uk/research/facilities/tick-cell-biobank/
Publications

PubMed=8812616; DOI=10.1006/jipa.1996.0050
Timothy J. Kurtti, Ulrike Gertrud Munderloh, Theodore George Andreadis, Louis Anthony Magnarelli, Thomas N. Mather;
Tick cell culture isolation of an intracellular prokaryote from the tick Ixodes scapularis.
J. Invertebr. Pathol. 67:318-321(1996)

Patent=US5869335
Ulrike Gertrud Munderloh, Timothy J. Kurtti, Katherine M. Kocan, Edmour F. Blouin, Sidney Alton Ewing;
Propagating bovine parasite by incubation of undifferentiated cells under low oxygen increased carbon dioxide, with organic buffers efficient, large scale production; diagnosis/vaccine for rocky mountains spotted fever.
Patent number US5869335, 09-Feb-1999

PubMed=16922866; DOI=10.1111/j.1462-5822.2006.00727.x
Vijayakrishna Singu, Lalitha Peddireddi, Kamesh R. Sirigireddy, Chuan-Min Cheng, Ulrike Gertrud Munderloh, Roman Reddy Ganta;
Unique macrophage and tick cell-specific protein expression from the p28/p30-outer membrane protein multigene locus in Ehrlichia chaffeensis and Ehrlichia canis.
Cell. Microbiol. 8:1475-1487(2006)

PubMed=17662657; DOI=10.1016/j.pt.2007.07.009
Lesley Bell-Sakyi, Erich Zweygarth, Edmour F. Blouin, Ernest Andrew Gould, Frans Jongejan;
Tick cell lines: tools for tick and tick-borne disease research.
Trends Parasitol. 23:450-457(2007)

PubMed=19242658; DOI=10.1007/s10493-009-9255-1; PMCID=PMC4465226
Sarah Arnao Billeter, Pedro Paulo V.P. Diniz, James M. Battisti, Ulrike Gertrud Munderloh, Edward B. Breitschwerdt, Michael G. Levy;
Infection and replication of Bartonella species within a tick cell line.
Exp. Appl. Acarol. 49:193-208(2009)

PubMed=22743047; DOI=10.1016/j.ttbdis.2012.05.002; PMCID=PMC3431536
Maria Pilar Alberdi, Matthew John Dalby, Julio Rodriguez-Andres, John K. Fazakerley, Alain Kohl, Lesley Bell-Sakyi;
Detection and identification of putative bacterial endosymbionts and endogenous viruses in tick cell lines.
Ticks Tick Borne Dis. 3:137-146(2012)

PubMed=25894426; DOI=10.1007/s10493-015-9908-1; PMCID=PMC4449809
Jonathan D. Oliver, Adela Sarahi Oliva Chavez, Roderick F. Felsheim, Timothy J. Kurtti, Ulrike Gertrud Munderloh;
An Ixodes scapularis cell line with a predominantly neuron-like phenotype.
Exp. Appl. Acarol. 66:427-442(2015)

PubMed=26424601; DOI=10.1074/mcp.M115.051938; PMCID=PMC4762615
Margarita Villar, Nieves Ayllon, Maria Pilar Alberdi, Andres Moreno, Maria Moreno, Raquel Tobes, Lourdes Mateos-Hernandez, Sabine Weisheit, Lesley Bell-Sakyi, Jose de la Fuente;
Integrated metabolomics, transcriptomics and proteomics identifies metabolic pathways affected by Anaplasma phagocytophilum infection in tick cells.
Mol. Cell. Proteomics 14:3154-3172(2015)

PubMed=29886187; DOI=10.1016/j.ttbdis.2018.05.015; PMCID=PMC6052676
Lesley Bell-Sakyi, Alistair C. Darby, Matthew Baylis, Benjamin L. Makepeace;
The Tick Cell Biobank: a global resource for in vitro research on ticks, other arthropods and the pathogens they transmit.
Ticks Tick Borne Dis. 9:1364-1371(2018)

PubMed=32158404; DOI=10.3389/fphys.2020.00152; PMCID=PMC7052283
Ahmed Al-Rofaai, Lesley Bell-Sakyi;
Tick cell lines in research on tick control.
Front. Physiol. 11:152.1-152.7(2020)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Nathalie Wurtz, Gwilherm Penant, Priscilla Jardot, Nathalie Duclos, Bernard La Scola;
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cross-references
Cell line collections (Providers) ATCC; CRL-3576
BEI_Resources; NR-12234
Encyclopedic resources Wikidata; Q54898276
Proteomic databases PRIDE; PXD002181
Entry history
Entry creation10-Apr-2015
Last entry update10-Apr-2025
Version number16