Cellosaurus U2OS MPX-Nomad NK2R (CVCL_YK71)
| Cell line name | U2OS MPX-Nomad NK2R |
|---|---|
| Synonyms | MPXNomad NK2R; MultiPleXed-Nomad NK2R U2OS |
| Accession | CVCL_YK71 |
| Resource Identification Initiative | To cite this cell line use: U2OS MPX-Nomad NK2R (RRID:CVCL_YK71) |
| Comments | Population: Caucasian. Characteristics: The green calcium-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a calmodulin-binding peptide fused to a KDEL signal. Characteristics: The red cAMP-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboFP650 fluorescent protein, a Ht31 peptide fused to a KDEL signal. Genetic integration: Method=Transfection/transduction; Gene=FPbase; JS838; TurboFP650 (Note=Far-red fluorescent protein derived from eqFP578). Genetic integration: Method=Transfection/transduction; Gene=FPbase; A315M; TurboGFP (Note=Green fluorescent protein from P.plumata). Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:11527; TACR2. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979. |
| Sequence variations |
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| Disease | Osteosarcoma (NCIt: C9145) Osteosarcoma (ORDO: Orphanet_668) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_YK69 (U2OS MPX-Nomad) |
| Sex of cell | Female |
| Age at sampling | 15Y |
| Category | Cancer cell line |
| Publications | PubMed=29412765; DOI=10.1177/2472630318754828 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q98133759 |
| Entry history | |
| Entry creation | 19-Dec-2019 |
| Last entry update | 14-Aug-2025 |
| Version number | 13 |