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Cellosaurus U2OS TACR1 calcium-Nomad (CVCL_YK58)

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Cell line name U2OS TACR1 calcium-Nomad
Synonyms Ca++ Nomad TACR1 (NK1)/U2OS; NK1 (TACR1) Nomad; NK1 Nomad
Accession CVCL_YK58
Resource Identification Initiative To cite this cell line use: U2OS TACR1 calcium-Nomad (RRID:CVCL_YK58)
Comments Population: Caucasian.
Characteristics: In absence of calcium, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad biosensor that promotes its cellular relocation in the vesicular trafficking of the cells.
Characteristics: The green calcium-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a calmodulin-binding peptide fused to a KDEL signal.
Transfected with: HGNC; 11526; TACR1.
Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (with p.Lys5Glu, p.Val117Met, p.Val149Asp, p.Val151Asp, p.Ala155Thr, p.Phe168Ser and p.Leu200Asp = TurboGFP).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Cell line collections (Providers) Innoprot; P70105
Encyclopedic resources Wikidata; Q98133764
Entry history
Entry creation19-Dec-2019
Last entry update05-Oct-2023
Version number9