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Cellosaurus CIPGC (CVCL_YJ30)

[Text version]
Cell line name CIPGC
Synonyms Conditional Immortal Porcine Granulosa Cells
Accession CVCL_YJ30
Resource Identification Initiative To cite this cell line use: CIPGC (RRID:CVCL_YJ30)
Comments Characteristics: Transduced with a P-TR3G-SV40largeT-T2A-mCherry-PhPGKp-PuroR-T2A-rTer-VP16 construct. The expression of the SV40 largeT antigen is under the control of the Tet-On system and induced by doxycycline.
Genetic integration: Method=Transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Ovary, ovarian follicle, granulosa cell layer; UBERON=UBERON_0005170.
Cell type: Granulosa cell; CL=CL_0000501.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Sex of cell Female
Age at sampling Age unspecified
Category Conditionally immortalized cell line
Publications

PubMed=31936362; DOI=10.3390/cells9010156; PMCID=PMC7017277
Yin-Shan Bai, Cui Zhu, Mei-Ying Feng, Bo Pan, Shou-Quan Zhang, Xiao-Shu Zhan, Hui-Fang Chen, Bing-Yun Wang, Ju-Lang Li;
Establishment of a reversibly inducible porcine granulosa cell line.
Cells 9:156.1-156.15(2020)

Cross-references
Encyclopedic resources Wikidata; Q93453606
Entry history
Entry creation12-Mar-2020
Last entry update14-Aug-2025
Version number10