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Cellosaurus MHHi006-A-1 (CVCL_XI78)

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Cell line name MHHi006-A-1
Synonyms CFTRdTomato74; hHSC_F1285_T-iPS2-CFTRTomato/wt C74; HSC1285iPS2_CFTRTomatoK74
Accession CVCL_XI78
Resource Identification Initiative To cite this cell line use: MHHi006-A-1 (RRID:CVCL_XI78)
Comments From: Hannover Medical School, LEBAO (MHH); Hannover; Germany.
Characteristics: Using TALEN a ZeoR-T2A-dTomato(nuc) construct was integrated in frame in front of the start codon of one of the two alleles of CFTR (PubMed=31473565).
Genetic integration: Method=TALEN; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Genetic integration: Method=TALEN; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_VN33 (MHHi006-A)
Sex of cell Male
Age at sampling <1D
Category Induced pluripotent stem cell
Publications

PubMed=31080112; DOI=10.1016/j.stemcr.2019.04.014; PMCID=PMC6565754
Sylvia Merkert, Madline Schubert, Ruth Olmer, Lena Engels, Silke Radetzki, Mieke Veltman, Bob J. Scholte, Janina Zollner, Nicoletta Pedemonte, Luis Juan Vicente Galietta, Jens P. von Kries, Ulrich Martin;
High-throughput screening for modulators of CFTR activity based on genetically engineered cystic fibrosis disease-specific iPSCs.
Stem Cell Reports 12:1389-1403(2019)

PubMed=31473565; DOI=10.1016/j.scr.2019.101542
Lena Engels, Ruth Olmer, Jeanne de la Roche, Gudrun Gohring, Saskia Ulrich, Ralf Haller, Ulrich Martin, Sylvia Merkert;
Generation of a CFTR knock-in reporter cell line (MHHi006-A-1) from a human induced pluripotent stem cell line.
Stem Cell Res. 40:101542-101542(2019)

Cross-references
Cell line databases/resources hPSCreg; MHHi006-A-1
Encyclopedic resources Wikidata; Q95989462
Entry history
Entry creation19-Dec-2019
Last entry update10-Sep-2024
Version number6