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Cellosaurus CF8Flp (CVCL_WZ18)

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Cell line name CF8Flp
Accession CVCL_WZ18
Resource Identification Initiative To cite this cell line use: CF8Flp (RRID:CVCL_WZ18)
Comments Characteristics: Contains a single recombination Flp-in target site for the stable integration and expression of a single cDNA, mini-gene, or complete gene.
Characteristics: Transfected with the pFRT/lacZeo plasmid. A single Flp recombination target (FRT) was integrated into chromosome 8.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSVori-).
Derived from site: In situ; Lung, bronchus, epithelium; UBERON=UBERON_0002031.
Cell type: Epithelial cell of bronchus; CL=CL_0002328.
Sequence variations
  • Mutation; HGNC; HGNC:1884; CFTR; Simple; p.Phe508del (c.1521_1523delCTT); ClinVar=VCV000007105; Zygosity=Homozygous (from parent cell line).
Disease Cystic fibrosis (NCIt: C2975)
Cystic fibrosis (ORDO: Orphanet_586)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_HL93 (CFBE41o-)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Transformed cell line
Web pages Provider; JHTV; C12966; https://jhu.technologypublisher.com/technology/23388
Publications

PubMed=26694805; DOI=10.1016/j.jcf.2015.11.010; PMCID=PMC4879073
Laura B. Gottschalk, Briana Vecchio-Pagan, Neeraj Sharma, Sangwoo T. Han, Arianna Franca, Elizabeth S. Wohler, Denise A.S. Batista, Loyal Andrew Goff, Garry R. Cutting;
Creation and characterization of an airway epithelial cell line for stable expression of CFTR variants.
J. Cyst. Fibros. 15:285-294(2016)

Cross-references
Encyclopedic resources Wikidata; Q93447351
Entry history
Entry creation06-Sep-2019
Last entry update10-Apr-2025
Version number12