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Cellosaurus H1-iCas9 (CVCL_WS14)

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Cell line name H1-iCas9
Synonyms H1 iCas9
Accession CVCL_WS14
Resource Identification Initiative To cite this cell line use: H1-iCas9 (RRID:CVCL_WS14)
Comments From: Memorial Sloan Kettering Cancer Center; New York; USA.
Characteristics: Cell line with robust Dox-inducible expression of Cas9. Can be used for rapid and highly efficient generation of biallelic knockout hPSCs for loss-of-function studies. Using TALEN two constructs SA-2A-Hygro-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele.
Genetic integration: Method=TALEN; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Miscellaneous: STR profile from personal communication of Huangfu, Dan-Wei.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): Direct_author_submission

Markers:
AmelogeninX,Y
CSF1PO12,13
D2S133818,19
D3S135815
D5S8189,11
D7S8208,12
D8S117912,13
D13S3178,11
D16S5399,13
D18S5117,18
D19S43313,15
D21S1128,32.2
FGA20,24
TH019.3
TPOX8,11
vWA15,17

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Publications

PubMed=28196600; DOI=10.1016/j.stem.2017.01.001; PMCID=PMC5419850
Zhong-Dong Shi, Kihyun Lee, Da-Peng Yang, Sadaf Amin, Nipun Verma, Qing V. Li, Zeng-Rong Zhu, Chew-Li Soh, Ritu Kumar, Todd Evans, Shui-Bing Chen, Dan-Wei Huangfu;
Genome editing in hPSCs reveals GATA6 haploinsufficiency and a genetic interaction with GATA4 in human pancreatic development.
Cell Stem Cell 20:675-688.e6(2017)

PubMed=31110351; DOI=10.1038/s41588-019-0408-9; PMCID=PMC6545159
Qing V. Li, Gary Dixon, Nipun Verma, Bess P. Rosen, Miriam Gordillo, Ren-He Luo, Chun-Long Xu, Qiong Wang, Chew-Li Soh, Da-Peng Yang, Miguel Crespo ...Show all 24 authors... , Abhijit Shukla, Qing Xiang, Friederike Dundar, Paul Zumbo, Matthew Witkin, Richard Patrick Koche, Doron Betel, Shui-Bing Chen, Joan Massague, Ralph Garippa, Todd Evans, Michael A. Beer, Dan-Wei Huangfu; Show fewer authors
Genome-scale screens identify JNK-JUN signaling as a barrier for pluripotency exit and endoderm differentiation.
Nat. Genet. 51:999-1010(2019)

Cross-references
Encyclopedic resources Wikidata; Q93935734
Entry history
Entry creation05-Jul-2019
Last entry update14-Aug-2025
Version number11