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Cellosaurus H1 iCas9 (CVCL_WS14)

[Text version]
Cell line name H1 iCas9
Accession CVCL_WS14
Resource Identification Initiative To cite this cell line use: H1 iCas9 (RRID:CVCL_WS14)
Comments From: Memorial Sloan Kettering Cancer Center; New York; USA.
Characteristics: Cell line with robust Dox-inducible expression of Cas9. Can be used for rapid and highly efficient generation of biallelic knockout hPSCs for loss-of-function studies. Using TALEN two constructs SA-2A-Hygro-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 locus, one in each allele.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Miscellaneous: STR profile from personal communication of Huangfu D.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): Direct_author_submission

Markers:
AmelogeninX,Y
CSF1PO12,13
D2S133818,19
D3S135815
D5S8189,11
D7S8208,12
D8S117912,13
D13S3178,11
D16S5399,13
D18S5117,18
D19S43313,15
D21S1128,32.2
FGA20,24
TH019.3
TPOX8,11
vWA15,17

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Publications

PubMed=28196600; DOI=10.1016/j.stem.2017.01.001
Shi Z.-D., Lee K., Yang D.-P., Amin S., Verma N., Li Q.V., Zhu Z.-R., Soh C.-L., Kumar R., Evans T., Chen S.-B., Huangfu D.-W.
Genome editing in hPSCs reveals GATA6 haploinsufficiency and a genetic interaction with GATA4 in human pancreatic development.
Cell Stem Cell 20:675-688.e6(2017)

PubMed=31110351; DOI=10.1038/s41588-019-0408-9
Li Q.V., Dixon G., Verma N., Rosen B.P., Gordillo M., Luo R.-H., Xu C.-L., Wang Q., Soh C.-L., Yang D.-P., Crespo M., Shukla A., Xiang Q., Dundar F., Zumbo P., Witkin M., Koche R., Betel D., Chen S.-B., Massague J., Garippa R., Evans T., Beer M.A., Huangfu D.-W.
Genome-scale screens identify JNK-JUN signaling as a barrier for pluripotency exit and endoderm differentiation.
Nat. Genet. 51:999-1010(2019)

Cross-references
Encyclopedic resources Wikidata; Q93935734
Entry history
Entry creation05-Jul-2019
Last entry update05-Oct-2023
Version number7