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Cellosaurus BU3 NKX2-1-GFP SFTPC-tdTomato (CVCL_WN82)

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Cell line name BU3 NKX2-1-GFP SFTPC-tdTomato
Synonyms BU3 NKX2-1GFP;SFTPCtdTomato; BU3-NGST; BU3 NGST; BU3NGST
Accession CVCL_WN82
Resource Identification Initiative To cite this cell line use: BU3 NKX2-1-GFP SFTPC-tdTomato (RRID:CVCL_WN82)
Comments From: Center for Regenerative Medicine; Boston; USA.
Population: Caucasian.
Characteristics: Using CRISPR/Cas9 a 2A-eGFP-pA was inserted after the NKX2-1 coding sequence and using TALENs a tdTomato-pA-loxP-PGKp-PuroR-pA-loxP was inserted at the SFTPC translational start site.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=TALEN; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Genetic integration: Method=TALEN; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_JW26 (CREM003i-BU3C2)
Sex of cell Male
Age at sampling 32Y
Category Induced pluripotent stem cell
Web pages Provider; CReM; 509; https://stemcellbank.bu.edu/Catalog/Item/Details/509
Publications

PubMed=28965766; DOI=10.1016/j.stem.2017.08.014; PMCID=PMC5755620
Anjali Jacob, Michael Morley, Finn Hawkins, Katherine B. McCauley, Jyh-Chang Jean, Hillary B. Heins, Cheng-Lun Na, Timothy E. Weaver, Marall Vedaie, Killian Hurley, Anne Hinds ...Show all 27 authors... , Scott J. Russo, Seunghyi Kook, William Zacharias, Matthias Ochs, Katrina Traber, Lee J. Quinton, Ana M. Crane, Brian R. Davis, Frances V. White, Jennifer Wambach, Jeffrey A. Whitsett, F. Sessions Cole, Edward E. Morrisey, Susan H. Guttentag, Michael F. Beers, Darrell N. Kotton; Show fewer authors
Differentiation of human pluripotent stem cells into functional lung alveolar epithelial cells.
Cell Stem Cell 21:472-488.e10(2017)

PubMed=31924806; DOI=10.1038/s41467-019-13916-6; PMCID=PMC6954238
Aditya Mithal, Amalia Capilla, Dar Heinze, Andrew Berical, Carlos Villacorta-Martin, Marall Vedaie, Anjali Jacob, Kristine Abo, Aleksander D. Szymaniak, Megan Peasley, Alexander Stuffer ...Show all 15 authors... , John Mahoney, Darrell N. Kotton, Finn Hawkins, Gustavo Mostoslavsky; Show fewer authors
Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells.
Nat. Commun. 11:215.1-215.15(2020)

Cross-references
Encyclopedic resources Wikidata; Q93433163
Entry history
Entry creation24-May-2019
Last entry update14-Aug-2025
Version number9