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Cellosaurus HeLa-EM2-11ht (CVCL_WN71)

[Text version]
Cell line name HeLa-EM2-11ht
Synonyms HeLa EM2-11ht
Accession CVCL_WN71
Resource Identification Initiative To cite this cell line use: HeLa-EM2-11ht (RRID:CVCL_WN71)
Comments Population: African American.
Characteristics: Stably expresses an improved version of the reverse tetracycline controlled transactivator (rtTA2S-M2) under the control of the human EF1a promoter. Also express an hygromycin B phosphotransferase-thymidine kinase (HygTk) fusion gene as a positive/negative selection marker.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

PubMed=19264799; DOI=10.1093/nar/gkp108; PMCID=PMC2673444
Ina Weidenfeld, Manfred Gossen, Rainer Low, David Kentner, Stefan Berger, Dirk Gorlich, Dusan Bartsch, Hermann Bujard, Kai Schonig;
Inducible expression of coding and inhibitory RNAs from retargetable genomic loci.
Nucleic Acids Res. 37:e50.1-e50.11(2009)

Cross-references
Encyclopedic resources Wikidata; Q94100654
Entry history
Entry creation24-May-2019
Last entry update10-Apr-2025
Version number9