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Cellosaurus BT-1 (CVCL_WK81)

[Text version]
Cell line name BT-1
Synonyms Bovine Trophoblast-1
Accession CVCL_WK81
Resource Identification Initiative To cite this cell line use: BT-1 (RRID:CVCL_WK81)
Comments Doubling time: ~2.72 days (Note=In fibroblast-conditioned medium), ~2.97 days (Note=In 10% FBS) (PubMed=11504534).
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Trophoblast; UBERON=UBERON_0000088.
Species of origin Bos taurus (Bovine) (NCBI Taxonomy: 9913)
Hierarchy Children:
CVCL_D5HN (BT-1S)
Sex of cell Sex unspecified
Age at sampling Blastocyst stage
Category Finite cell line
Publications

PubMed=11504534; DOI=10.1053/plac.2001.0702
Shimada A., Nakano H., Takahashi T., Imai K., Hashizume K.
Isolation and characterization of a bovine blastocyst-derived trophoblastic cell line, BT-1: development of a culture system in the absence of feeder cell.
Placenta 22:652-662(2001)

PubMed=15613779; DOI=10.1262/jrd.16072
Ushizawa K., Takahashi T., Kaneyama K., Tokunaga T., Tsunoda Y., Hashizume K.
Gene expression profiles of bovine trophoblastic cell line (BT-1) analyzed by a custom cDNA microarray.
J. Reprod. Dev. 51:211-220(2005)

PubMed=16251743; DOI=10.1385/1-59259-983-4:177
Hashizume K., Shimada A., Nakano H., Takahashi T.
Bovine trophoblast cell culture systems: a technique to culture bovine trophoblast cells without feeder cells.
Methods Mol. Med. 121:179-188(2006)

PubMed=22632112; DOI=10.1186/1477-7827-10-41; PMCID=PMC3419082
Koshi K., Suzuki Y., Nakaya Y., Imai K., Hosoe M., Takahashi T., Kizaki K., Miyazawa T., Hashizume K.
Bovine trophoblastic cell differentiation and binucleation involves enhanced endogenous retrovirus element expression.
Reprod. Biol. Endocrinol. 10:41.1-41.11(2012)

PubMed=24950590; DOI=10.1186/1477-7827-12-55; PMCID=PMC4078357
Awad M., Koshi K., Kizaki K., Takahashi T., Hashizume K.
SOLD1 is expressed in bovine trophoblast cell lines and regulates cell invasiveness.
Reprod. Biol. Endocrinol. 12:55.1-55.10(2014)

Cross-references
Encyclopedic resources Wikidata; Q93432653
Entry history
Entry creation24-May-2019
Last entry update10-Apr-2025
Version number8