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Cellosaurus OP27 (CVCL_WI58)

[Text version]
Cell line name OP27
Synonyms OP-27; Olfactory Placode 27
Accession CVCL_WI58
Resource Identification Initiative To cite this cell line use: OP27 (RRID:CVCL_WI58)
Comments Characteristics: At the permissive temperature (33 Celsius) expresses a transcriptional profile representing intermediate-late developmental stages in the olfactory receptor neuron (ORN) lineage. At the non-permissive temperature (39 Celsius) can be induced by all-trans retinoic acid to differentiate into a population of mature bipolar olfactory receptor neuron-like cells (CelloPub=CLPUB00748; PubMed=12093156).
Doubling time: 52 hours (Note=At 33 Celsius) (CelloPub=CLPUB00748; PubMed=12093156).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).
Caution: PubMed=10630978 incorrectly describes this cell line as originating from a mouse neuroblastoma.
Derived from site: In situ; Olfactory placode; UBERON=UBERON_0003050.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3H.
Sex of cell Sex unspecified
Age at sampling 10-11FD
Category Conditionally immortalized cell line
Publications

CLPUB00748
Sihaam Boolay;
Immortalisation, characterisation and differentiation of temperature sensitive cell lines from the olfactory neuroepithelium.
Thesis PhD (1998); University of Cape Town; Cape Town; South Africa

PubMed=10630978; DOI=10.1667/0033-7587(2000)153[0062:FORIMI]2.0.CO;2
John M. Akudugu, Jacobus Petrus Slabbert, Antonio Mendes Serafin, Lothar Bohm;
Frequency of radiation-induced micronuclei in neuronal cells does not correlate with clonogenic survival.
Radiat. Res. 153:62-67(2000)

PubMed=12093156; DOI=10.1006/mcne.2002.1106
Nicola Illing, Sihaam Boolay, Jenny S. Siwoski, Diana Casper, Mary Theresa Lucero, Angela Jane Roskams;
Conditionally immortalized clonal cell lines from the mouse olfactory placode differentiate into olfactory receptor neurons.
Mol. Cell. Neurosci. 20:225-243(2002)

Cross-references
Anatomy/cell type resources BTO; BTO_0006226
Encyclopedic resources Wikidata; Q98128349
Entry history
Entry creation24-May-2019
Last entry update14-Aug-2025
Version number11