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Cellosaurus A-375 NRAS p.Q61K-Luc2 (CVCL_VR65)

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Cell line name A-375 NRAS p.Q61K-Luc2
Synonyms NRAS-mutant-A375 Isogenic-Luc2
Accession CVCL_VR65
Resource Identification Initiative To cite this cell line use: A-375 NRAS p.Q61K-Luc2 (RRID:CVCL_VR65)
Comments Population: Caucasian.
Characteristics: Shows significant resistance to the BRAF inhibitors Dabrafineb and Vemurafenib when compared to its parental cell line in 2D and 3D culture systems. Can be a useful model to study the RAS-RAF-MEK-ERK-MAP kinase signaling pathway and to screen potential BRAF inhibitors and anti-cancer compounds for drug discovery and development (ATCC=CRL-1619IG-2-LUC2).
Characteristics: Stably expresses firefly luciferase under the control of the human EF1a promoter.
Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase (Note=With optimized codon usage for mammalian expression = Luc2).
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Sequence variations
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Glu61Ter (c.181G>T) (p.Gly75Val, c.224G>T); ClinVar=VCV000376309; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Glu69Ter (c.205G>T) (p.Gly83Val, c.248G>T); ClinVar=VCV000376308; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple_edited; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Homozygous; Note=By CRISPR/Cas9 (ATCC=CRL-1619IG-2-LUC2).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-146C>T (c.250C>T) (C250T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_JF22 (A-375 NRAS p.Q61K)
Sex of cell Female
Age at sampling 54Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-1619IG-2-LUC2

Markers:
AmelogeninX
CSF1PO11,12
D5S81812
D7S8209
D13S31711,14
D16S5399
TH018
TPOX8,10
vWA16,17

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Cross-references
Cell line collections (Providers) ATCC; CRL-1619IG-2-LUC2
Cell line databases/resources cancercelllines; CVCL_VR65
Encyclopedic resources Wikidata; Q93311033
Entry history
Entry creation25-Feb-2019
Last entry update14-Aug-2025
Version number15