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Cellosaurus Elf1-iCas9 (CVCL_VR49)

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Cell line name Elf1-iCas9
Accession CVCL_VR49
Resource Identification Initiative To cite this cell line use: Elf1-iCas9 (RRID:CVCL_VR49)
Comments Characteristics: Cell line with robust Dox-inducible expression of Cas9. Can be used for rapid and highly efficient generation of biallelic knockout hPSCs for loss-of-function studies.
Characteristics: Using TALEN two constructs SA-2A-PuroR-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele.
Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=TALEN; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C115 (Elf1)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=29466914; DOI=10.1080/15384101.2018.1442621; PMCID=PMC5969550
Amy Ana Ferreccio, Julie Mathieu, Damien Detraux, Logeshwaran Somasundaram, Christopher Cavanaugh, Bryce Sopher, Karin Fischer, Thomas Bello, Abdiasis M. Hussein, Shiri Levy, Savannah Cook ...Show all 21 authors... , Sonia B. Sidhu, Filippo Artoni, Nathan J. Palpant, Hans Reinecke, Yu-Liang Wang, Patrick J. Paddison, Charles E. Murry, Suman Jayadev, Carol B. Ware, Hannele Ruohola-Baker; Show fewer authors
Inducible CRISPR genome editing platform in naive human embryonic stem cells reveals JARID2 function in self-renewal.
Cell Cycle 17:535-549(2018)

Cross-references
Encyclopedic resources Wikidata; Q93539988
Entry history
Entry creation13-Nov-2018
Last entry update14-Aug-2025
Version number10