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Cellosaurus Elf1-iCas9 (CVCL_VR49)

[Text version]
Cell line name Elf1-iCas9
Accession CVCL_VR49
Resource Identification Initiative To cite this cell line use: Elf1-iCas9 (RRID:CVCL_VR49)
Comments Characteristics: Cell line with robust Dox-inducible expression of Cas9. Can be used for rapid and highly efficient generation of biallelic knockout hPSCs for loss-of-function studies.
Characteristics: Using TALEN two constructs SA-2A-PuroR-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele.
Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=TALEN; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C115 (Elf1)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=29466914; DOI=10.1080/15384101.2018.1442621; PMCID=PMC5969550
Ferreccio A., Mathieu J., Detraux D., Somasundaram L., Cavanaugh C., Sopher B., Fischer K., Bello T., Hussein A.M., Levy S., Cook S., Sidhu S.B., Artoni F., Palpant N.J., Reinecke H., Wang Y.-L., Paddison P.J., Murry C.E., Jayadev S., Ware C.B., Ruohola-Baker H.
Inducible CRISPR genome editing platform in naive human embryonic stem cells reveals JARID2 function in self-renewal.
Cell Cycle 17:535-549(2018)

Cross-references
Encyclopedic resources Wikidata; Q93539988
Entry history
Entry creation13-Nov-2018
Last entry update10-Sep-2024
Version number8